
David Friedman
I previously have managed a research project involving multiple collaborators, with a focusing on using RNA-seq to analyze... | Tallahassee, Florida, United States
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David Friedman’s Location Tallahassee, Florida, United States
David Friedman’s Expertise I previously have managed a research project involving multiple collaborators, with a focusing on using RNA-seq to analyze blueberry fruit tissue to identify key candidate genes relating to the ‘crisp’ texture trait identified in the UF blueberry breeding germplasm. This included both field work and laboratory work. The field research consisted of working outside in the research material’s field located on a farm in Waldo, FL, both individually and as the head of a small team to tag and re-tag plants, check plant health and field conditions, and harvesting fruit for downstream research use. I also performed logistical work before each trip, consisting of organizing and providing necessary supplies/tools for each trip and arranging transportation. Laboratory work consisted of collecting and handling field samples, including cataloging, measurement of qualities such as weight, degree of ripeness, compression force, and puncture force, freezing samples for long-term storage, processing samples for scientific experiments, and performing said scientific experiments related to my research topic. Skills obtained over the course of research include DNA and RNA extraction, DNA/cDNA amplification using PCR, several DNA/cDNA quantification methods, sample preparatory methods for DNA/cDNA sequencing, computational data analysis, and plant tissue culture, as well as additional laboratory equipment proficiencies. Laboratory equipment proficiencies obtained over the course of the project include gel electrophoresis apparatus, water baths, programmable thermocyclers, -20oC and -80oC freezers, liquid nitrogen canisters, spectrophotometers (including Agilent nanodrop bioanalyzer), Firmtec 2 fruit texture analyzers, TA.HDPlus Connect texture analyzer, autoclaves (both digitally controlled and manual), light microscopes, and incubators. Other aspects of my work consisted of laboratory management and greenhouse management. Laboratory management consisted of reporting need for additional supplies, testing and calibrating equipment and reporting equipment malfunction, assisting with setting up new lab equipment, assisting with storage of samples and storage reorganization, and correct storage/disposal of hazardous waste chemicals within lab prior to pick-up. Greenhouse work consisted of learning details of specific plant species living requirements, formulating soil composition prior to potting, potting, watering, replanting, tissue culture and cutting propagation work, and pesticide application.
David Friedman’s Current Industry Us Department Of Agriculture Agricultural Research Service
David
Friedman’s Prior Industry
University Of Florida
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Us Department Of Agriculture Agricultural Research Service
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Work Experience

Us Department Of Agriculture Agricultural Research Service
Biological Science Laboratory Technician
Fri Apr 01 2022 00:00:00 GMT+0000 (Coordinated Universal Time) — Present
University Of Florida
Research Assistant
Sat Aug 01 2015 00:00:00 GMT+0000 (Coordinated Universal Time) — Sat Feb 01 2020 00:00:00 GMT+0000 (Coordinated Universal Time)