Jakub Baudys

FAQ About Jakub Baudys

• What is Jakub Baudys's address?

  Jakub Baudys is located at
  Lilburn, Georgia, United States

• What is Jakub Baudys's job title?

  Jakub Baudys is a
  ●Detection potentially positive clinical samples using peptide substrate assays. ●Development of bacterial toxin peptide substrate assays for detection using clinical matrices. ●Basic research on the kinetics of bacterial toxins with protein/peptide substrates. ●1D-PAGE analysis for bottom-up proteomics from plasma. ●Strain identification of infectious organisms by affinity capture, proteomics approach using the Orbitrap-Elite. ●Discovery of toxin cleavage sites using TOF-MS of the intact protein substrate and cleavage products. ●Top-down proteomics using the OrbiTrap-Elite. ●Assisted with the successful IND filing of Tmab-DM1, antibody-drug conjugate Method Development and Technology Transfer ●Development of bioanalytical assays for the metabolites of antibody drug conjugates, analytes under 2 kDa and above 25 kDa. Developed methods for the Prospekt II On-Line SPE system. ●Led the efforts to introduce a high degree of autonomous/robotic instrumentation into the MS group. Automated methods using the Hamilton StarLet system and LC/MS/MS. ●Technical contact for the outsourcing and oversight of GLP clinical and non-clinical studies to CROs. Assisted in method transfer, method troubleshooting. ●Characterization of late stage research monoclonal antibody immunoconjugates (antibody-drug conjugate, ADC) by capillary-ESI/LC/MS-TOF. Predicted the attachment of conjugates to specific sites on MAbs through site specific cleavage of intact MAbs and analysis by mass spectrometry. ●Methods for binding specific antigens to affinity supports, affinity/immunoaffinity chromatography. ●Use of affinity binding techniques to recover ADCs from plasma to determine their structure during the PK cycle. ●Sequencing of peptides using the LTQ linear ion trap and MS2 and MS3 to confirm the sequence of residues in peptides. ●Development of bottom-up proteomics techniques to sequence antibody drug conjugates and determine the presence of metabolites in plasma. Specialties: AP Biosystems 4800+ MALDI-TOF, LTQ-FT, LC/MS/MS (triple quad, hybrid TOF, linear ion trap). Instruments: AP Biosystems API 4000 QTRAP, Synapt HDMS QTOF, API QSTAR XL MS/TOF, Prospekt II SPE, Hamilton StarLet. Software: Analyst, Watson LIMS, Excalibur, Agilent Chem Station. On-line liquid and solid phase extraction techniques. HPLC method development: reverse phase, polymeric and size exclusion. Ligand binding to affinity supports. Capillary-ION spray (ESI) and capillary flow HPLC. Centers For Disease Control And Prevention

• What industry does Jakub Baudys currently work in?

  Jakub Baudys currently works in the
  Centers For Disease Control And Prevention

• What is Jakub Baudys's area of expertise?

  Jakub Baudys specializes in
  ●Detection potentially positive clinical samples using peptide substrate assays. ●Development of bacterial toxin peptide substrate assays for detection using clinical matrices. ●Basic research on the kinetics of bacterial toxins with protein/peptide substrates. ●1D-PAGE analysis for bottom-up proteomics from plasma. ●Strain identification of infectious organisms by affinity capture, proteomics approach using the Orbitrap-Elite. ●Discovery of toxin cleavage sites using TOF-MS of the intact protein substrate and cleavage products. ●Top-down proteomics using the OrbiTrap-Elite. ●Assisted with the successful IND filing of Tmab-DM1, antibody-drug conjugate Method Development and Technology Transfer ●Development of bioanalytical assays for the metabolites of antibody drug conjugates, analytes under 2 kDa and above 25 kDa. Developed methods for the Prospekt II On-Line SPE system. ●Led the efforts to introduce a high degree of autonomous/robotic instrumentation into the MS group. Automated methods using the Hamilton StarLet system and LC/MS/MS. ●Technical contact for the outsourcing and oversight of GLP clinical and non-clinical studies to CROs. Assisted in method transfer, method troubleshooting. ●Characterization of late stage research monoclonal antibody immunoconjugates (antibody-drug conjugate, ADC) by capillary-ESI/LC/MS-TOF. Predicted the attachment of conjugates to specific sites on MAbs through site specific cleavage of intact MAbs and analysis by mass spectrometry. ●Methods for binding specific antigens to affinity supports, affinity/immunoaffinity chromatography. ●Use of affinity binding techniques to recover ADCs from plasma to determine their structure during the PK cycle. ●Sequencing of peptides using the LTQ linear ion trap and MS2 and MS3 to confirm the sequence of residues in peptides. ●Development of bottom-up proteomics techniques to sequence antibody drug conjugates and determine the presence of metabolites in plasma. Specialties: AP Biosystems 4800+ MALDI-TOF, LTQ-FT, LC/MS/MS (triple quad, hybrid TOF, linear ion trap). Instruments: AP Biosystems API 4000 QTRAP, Synapt HDMS QTOF, API QSTAR XL MS/TOF, Prospekt II SPE, Hamilton StarLet. Software: Analyst, Watson LIMS, Excalibur, Agilent Chem Station. On-line liquid and solid phase extraction techniques. HPLC method development: reverse phase, polymeric and size exclusion. Ligand binding to affinity supports. Capillary-ION spray (ESI) and capillary flow HPLC.

• Which languages does Jakub Baudys speak? 

  Jakub Baudys speaks the following languages:
  No languages available.

• Which industries has Jakub Baudys previously worked in?   

  Jakub Baudys has prior experience in the following industries:
  Genentech , Tandemlabs

• What is Jakub Baudys’s work experience?  

  Jakub Baudys has the following professional experience:
  Centers For Disease Control And Prevention , Genentech , Tandemlabs .

• How can I find Jakub Baudys on LinkedIn?  

  You can view Jakub Baudys’s
  LinkedIn profile at LinkedIn URL.

• What are Jakub Baudys’s work email addresses?  

  Jakub Baudys’s work email addresses are
  [email protected] .

• What company does Jakub Baudys work for?  

  Jakub Baudys works at
  Centers For Disease Control And Prevention