Subhash Chand

FAQ About Subhash Chand

• What is Subhash Chand's address?

  Subhash Chand is located at
  Omaha, Nebraska, United States

• What is Subhash Chand's job title?

  Subhash Chand is a
  Key Skillsets: Microbiology/aseptic Techniques: Mammalian cell culture, Isolation of PBMC, serum and plasma isolation, Exosome/Extracellular Biology, Vaccine Design, media preparation, and mice handling. Analytical Techniques: Multicolor flow cytometry, Electron Microscopy, Confocal Microscopy, sample staining and acquisition, Elispot/ Luminex-based assays for cytokine/chemokine assessments. Proteomics Techniques: Recombinant protein expression, purification, SDS-PAGE, western blotting, ELISA, Strep-tag, His-tag, HPLC, GC, GC-MS, column chromatography, Ion exchange, protein dialysis, Akta Prime, Gel filtration, enzyme assay, Fluorescence spectroscopy, UV-vis microplate reader, stopped-flow, and UV-vis. Molecular Biology Techniques: Primer designing, DNA-extraction, Gene cloning, restriction digestion, gel elec-trophoresis, gel extraction, ligation, PCR, site-directed mutagenesis, DNA sequence analysis, plasmid trans-formation, and quantification of nucleic acids using Nanodrop. Computer Skills: Flow Jo, GraphPad Prism, Photoshop, Illustrator, PyMOL, VMD viewer, BioEdit, BLAST, NCBI, sequence alignment, pDRAW, SnapGene Viewer, Vector NTI, Chem draw, SciFinder, Sigma plot, MS office, Excel, Word, and Power-Point. RESEARCH EXPERIENCE  Role of Exosome/Extracellular Vesicle in pathogenesis and as Biomarkers. Tissue derived exosome for organs specific exosome markers. Exosome, HIV, Drug of abuse and Neurological Disorders. Investigating the impact of HIV infection on innate immune in HIV infected patients. α47 integrin present on host cells recognizes the V1V2 domain of the HIV-1 envelope protein. By biochemical and mutational analysis, we showed that glycosylation is a key modulator of V1V2 conformation and binding to α47 We investigate that gp120 monomer, and scaffolded gp16-V1V2 envelopes induce robust α4β7 blocking Abs which significantly blocked α4β7: HIV envelope protein interaction. Generated fully functional protein microarrays, with monolayer arrangements and complete control over their orientations, using recombinant proteins produced by introducing non-canonical amino ac-id by site-directed mutagenesis. Showed the potential of unnatural amino acids to enable non-natural functions when incorporated in the protein scaffold of heme metalloproteins. University Of Nebraska Medical Center

• What industry does Subhash Chand currently work in?

  Subhash Chand currently works in the
  University Of Nebraska Medical Center

• What is Subhash Chand's area of expertise?

  Subhash Chand specializes in
  Key Skillsets: Microbiology/aseptic Techniques: Mammalian cell culture, Isolation of PBMC, serum and plasma isolation, Exosome/Extracellular Biology, Vaccine Design, media preparation, and mice handling. Analytical Techniques: Multicolor flow cytometry, Electron Microscopy, Confocal Microscopy, sample staining and acquisition, Elispot/ Luminex-based assays for cytokine/chemokine assessments. Proteomics Techniques: Recombinant protein expression, purification, SDS-PAGE, western blotting, ELISA, Strep-tag, His-tag, HPLC, GC, GC-MS, column chromatography, Ion exchange, protein dialysis, Akta Prime, Gel filtration, enzyme assay, Fluorescence spectroscopy, UV-vis microplate reader, stopped-flow, and UV-vis. Molecular Biology Techniques: Primer designing, DNA-extraction, Gene cloning, restriction digestion, gel elec-trophoresis, gel extraction, ligation, PCR, site-directed mutagenesis, DNA sequence analysis, plasmid trans-formation, and quantification of nucleic acids using Nanodrop. Computer Skills: Flow Jo, GraphPad Prism, Photoshop, Illustrator, PyMOL, VMD viewer, BioEdit, BLAST, NCBI, sequence alignment, pDRAW, SnapGene Viewer, Vector NTI, Chem draw, SciFinder, Sigma plot, MS office, Excel, Word, and Power-Point. RESEARCH EXPERIENCE  Role of Exosome/Extracellular Vesicle in pathogenesis and as Biomarkers. Tissue derived exosome for organs specific exosome markers. Exosome, HIV, Drug of abuse and Neurological Disorders. Investigating the impact of HIV infection on innate immune in HIV infected patients. α47 integrin present on host cells recognizes the V1V2 domain of the HIV-1 envelope protein. By biochemical and mutational analysis, we showed that glycosylation is a key modulator of V1V2 conformation and binding to α47 We investigate that gp120 monomer, and scaffolded gp16-V1V2 envelopes induce robust α4β7 blocking Abs which significantly blocked α4β7: HIV envelope protein interaction. Generated fully functional protein microarrays, with monolayer arrangements and complete control over their orientations, using recombinant proteins produced by introducing non-canonical amino ac-id by site-directed mutagenesis. Showed the potential of unnatural amino acids to enable non-natural functions when incorporated in the protein scaffold of heme metalloproteins.

• Which languages does Subhash Chand speak? 

  Subhash Chand speaks the following languages:
  No languages available.

• Which industries has Subhash Chand previously worked in?   

  Subhash Chand has prior experience in the following industries:
  University Of Nebraska Medical Center , University Of Nebraska Medical Center , University Of Miami Miller School Of Medicine , The Catholic University Of America , University Of Texas At Arlington , Meerut Institute Of Technology

• What is Subhash Chand’s work experience?  

  Subhash Chand has the following professional experience:
  University Of Nebraska Medical Center , University Of Nebraska Medical Center , University Of Nebraska Medical Center , University Of Miami Miller School Of Medicine , The Catholic University Of America , University Of Texas At Arlington , Meerut Institute Of Technology .

• How can I find Subhash Chand on LinkedIn?  

  You can view Subhash Chand’s
  LinkedIn profile at LinkedIn URL.

• What are Subhash Chand’s work email addresses?  

  Subhash Chand’s work email addresses are
  [email protected] .

• What company does Subhash Chand work for?  

  Subhash Chand works at
  University Of Nebraska Medical Center